Effects of hypersensitivity and induced radioresistance in CHO-K1 cells following exposure to gamma-rays and accelerated carbon ions

«Radiation and Risk», 2021, vol. 30, No. 4, pp.156-167

10.21870/0131-3878-2021-30-4-156-167

Authors

Koryakina E.V. – Sen. Res., C. Sc., Biol. Contacts: 4 Korolev Str., Obninsk, Kaluga region, Russia, 249035. Tel.: (484) 399-72-76; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Potetnya V.I. – Sen. Res., C. Sc., Biol.
Troshina M.V. – Researcher
Baykuzina R.M. – Lead. Engineer
Koryakin S.N. – Head of Dep., C. Sc., Biol. A. Tsyb MRRC.
Kaprin A.D. – Director General, Academician of RAS, MD, Prof. NMRRC.
¹ A. Tsyb MRRC, Obninsk
² NMRRC, Moscow

Abstract

The study presents results of investigations on chromosome aberrations (CA) yield in Chinese ham-ster ovary cells after exposure to gamma-rays and accelerated carbon ions (455 MeV/amu) at doses less than 1 Gy in the pristine Bragg curve plateau and behind the Bragg peak, where normal tissues are situated in the course of radiotherapy. Initial parts of dose curves for total CA and terminal deletions frequencies differed from linear-quadratic dependence and the region of induced radioresistance were observed at 0.1-0.6 Gy for gamma-rays. The similar curve shapes were detected for carbon ions, but plateau region was shorter (0.15-0.35 Gy). Despite the uniform shape of the dose curves for the CA yield, a definite dependence of the cytogenetic effect on the linear energy transfer (LET) is observed. The CA frequency increased with LET rising in the order: gamma-irradiation (0.2 keV/μm), carbon ions at the Bragg curve plateau (10-12 keV/μm) and at the «tail» of Bragg peak (25-27 keV/μm). Outside this range, the yield of chromosome aberrations also enhanced with increasing LET in the same order. The results obtained confirm that the hypersensitivity and induced radioresistance phenomena are characteristic for low level exposure to low-LET and middle-LET radiations when chromosome aberration test is used.

Key words
accelerated carbon ions, chromosome aberrations, Chinese Hamster Ovary cells, hypersensitivity, induced radioresistance, linear energy transfer.

References

1. Ou H.F., Zhang B., Zhao S.J. Monte Carlo simulation for calculation of fragments produced by 400 MeV/u carbon ion beam in water. Nucl. Instrum. Methods Phys. Res. B, 2017, vol. 396, pp. 18-25.

2. Tommasino F., Durante M. Proton radiobiology. Cancers, 2015, vol. 7, no. 1, pp. 353-381.

3. Marples B., Joiner M.C. The response of Chinese hamster V79 cells to low radiation doses: evidence of enhanced sensitivity of the whole cell population. Radiat. Res. 1993, vol. 133, no. 1, pp. 41-51.

4. Marples B., Lam G.K.Y., Zhou H., Skov K.A. The response of Chinese hamster V79-379A cells exposed to negative pi-mesons: evidence that increased radioresistance is dependent on linear energy transfer. Radiat. Res., 1994, vol. 138, no. 1 Suppl., pp. S81-S84.

5. Koryakina E.V., Potetnya V.I., Troshina M.V., Efimova M.N., Baykuzina R.M., Koryakin S.N., Lychagin A.A., Pikalov V.A., Ulyanenko S.E. Comparison of the biological efficiency of accelerated carbon ions and heavy recoils in Chinese hamster cells. Radiatsiya i risk – Radiation and Risk, 2019, vol. 28, no. 3, pp. 96-106. (In Russian).

6. Freshney R.J. Kul'tura zhivotnyh kletok. Prakticheskoe rukovodstvo [Culture of animal cells. A manual of basic technique. Fifth Edition]. Moscow, BINOM, 2010. 691 p.

7. Matsuya Y., McMahon S.J., Tsutsumi K., Sasaki K., Okuyama G., Yoshii Y., Mori R., Oikawa J., Prise K.M., Date H. Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates. Scientific Reports, 2018, vol. 8, no. 1, pp. 1-14.

8. Obaturov G.M., Potetnya V.I. Chromosome aberrations and reproductive death of mammalian cells: quanti-tative correlation between these effects. Radiobiologiya – Radiobiology, 1986, vol. 26, no. 4, pp. 465-472. (In Russian).

9. Conforth M.N., Bedford J.S. A quantitative comparison of potentially lethal damage repair and the rejoining of interphase chromosome breaks in low passage normal human fibroblasts. Radiat. Res., 1987, vol. 111, no. 3, pp. 385-405.

10. Geras’kin S.A., Oudalova A.A., Kim J.K., Dikarev V.G., Dikareva N.S. Cytogenetic effect of low dose -radiation in Hordeum vulgare seedlings: non-linear dose-effect relationship. Radiat. Environ. Biophys., 2007, vol. 46, no. 1, pp. 31-41.

11. Lloyd D.C., Edwards A.A., Leonard A., Deknudt G.L., Verschaeve L., Natarajan A.T., Darroudi F., Obe G., Palitti F., Tanzarellae C., Tawn E.J. Chromosomal aberrations in human lymphocytes induced in vitro by very low doses of X-rays. Int. J. Radiat. Biol., 1992, vol. 61, no. 3, pp. 335-343.

12. Sevan'kaev A.V., Luchnik N.V. Effect of gamma-irradiation on human chromosomes. VIII. Cytogenetic effect of low doses during in vitro irradiation. Genetika – Genetics, 1977, vol. 13, no. 3, pp. 524-532. (In Russian).

13. Sevan’kaev A.V., Khvostunov I.K., Potetnya V.I. The low dose and low dose rate cytogenetic effects induced by -radiation in human blood lymphocytes in vitro. II. The results of cytogenetic study. Radiacionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 2012, vol. 52, no. 1, pp. 11-24. (In Russian).

14. Martin L.M., Marples B., Lynch T.H., Hollywood D., Marignol L. Exposure to low dose ionising radiation: molecular and clinical consequences. Cancer Lett., 2014, vol. 349, no. 1, pp. 98-106.

15. Seth I., Joiner M.C., Tucker J.D. Cytogenetic low-dose hyper-radiosensitivity is observed in human peripheral blood lymphocytes. Int. J. Radiat. Oncol. Biol. Phys., 2015, vol. 91, no. 1, pp. 82-90.

16. Schettino G., Folkard M., Prise K.M., Vojnovic B., Bowey A.G., Michael B.D. Low-dose hypersensitivity in Chinese hamster V79 cells targeted with counted protons using a charged-particle microbeam. Radiat. Res., 2001, vol. 156, pp. 526-534.

17. Koryakina E.V., Potetnya V.I. Neutron low doses cytogenetic effects in mammalian cells. Al’manakh klinich-eskoy meditsiny – Almanac of Clinical Medicine, 2015, vol. 41, pp. 72-78. (In Russian).

18. Dionet C., Tchirkov A., Alard J.P., Arnold J., Dhermain J., Rapp M., Bodez V., Tamain J.C., Monbel I., Malet P., Kwiatkowski F., Donnarieix D., Veyre A., Verrelle P. Effects of low-dose neutrons applied at reduced dose rate on human melanoma cells. Radiat. Res., 2000, vol. 154, no. 4, pp. 406-411.

19. Xue L., Yu D., Furusawa Y., Cao J., Okayasu R., Fan S. ATM-dependent hyper-radiosensitivity in mamma-lian cells irradiated by heavy ions. Int. J. Radiat. Oncol. Biol. Phys., 2009, vol. 75, no. 1, pp. 235-243.

20. Marples B., Skov K.A. Small doses of high-linear energy transfer radiation increase the radioresistance of Chinese hamster V79 cells to subsequent X irradiation. Radiat. Res., 1996, vol. 146, no. 4, pp. 382-387.

21. Koryakina Е.V., Potetnya V.I., Klykov S.A., Pugachev R.M. Biological efficiency of neutrons and alpha radiation with a comparable LET. Aktual'nye problemy gumanitarnyh i estestvennyh nauk – Actual Problems of the Humanities and Natural Sciences, 2013, vol. 12, no. 59, part III, pp. 39-40. (In Russian).

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