The effect of low dose-rate radiation on thiol-disulfide system and lipid antioxidants in blood plasma of exposed persons

«Radiation and Risk», 2017, vol. 26, No. 4, pp.111-123

DOI: 10.21870/0131-3878-2017-26-4-111-123


Ivanenko G.F. – Senior Researcher, C. Sc., Biol. Emanuel Institute of Biochemical Physics of RAS (IBCP RAS), Moscow Contacts: 4 Kosygin str., Moscow, Russia, 119334. Tel.: 8 (495) 939-74-75, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Burlakova E.B. – Prof., D. Sc., Biol. Emanuel Institute of Biochemical Physics of RAS (IBCP RAS), Moscow.


Understanding of mechanisms of non-specific resistance to damaging agents, such as low dose-rate ionizing radiation, is an area of our interest. Redox status of the glutathione system (GSH, GSSG) and lipid antioxidants, vitamins E and A, in the blood plasma of people exposed to low dose-rate radiation following the Chernobyl accident was examined in 4, 5, 6, 7 and 20 years after the exposure. Obtained data demonstrate complex interactions between watersoluble and lipid antioxidants in blood plasma of exposed people of different ages. The antioxiadant systems respond variously to low dose radiation (0.1-20 cSv) and high doses radiation (20-150 cSv), Exposure to low doses of low-dose-rate radiation may produce more pronounced effects on redox balance as compared with effects from exposure to high dose radiation of the same dose-rate. Different effects of low and high radiation doses on antioxidant systems can be due to sensibilization of these systems to radiation caused by damage of regulatory interactions in the processes, in which free radicals participate. The alteration of glutathione reduced and oxidized forms ratio due to significant increase in the GSH concentration (a biomarker of "harm") in blood plasma in people exposed to low dose radiation of low dose-rate can be critical, when glutathione antioxidant transforms into glutathione pro-oxidant.

Key words
Reduced glutathione, oxidized glutathione, thiol-disulfide ratio, vitamin E, vitamin A, blood plasma, lymphocytes, 137Cs, low-intensity radiation, population living in contaminated territories, liquidators, mice of different lines.


1. Morgan W.F., Schwartz J.L. Environmental mutagen society symposium on risks of low dose, low dose rate exposures of ionizing radiation to humans. Int. J. Radiat. Biol., 2007, vol. 83, pp. 491-499.

2. Crompton N.E.A. Programmed cell response to ionizing radiation damage. Acta Oncologica, 1998, vol. 37, pp. 129-142.

3. Govorun R.D. Cytogenetic damage and mutagenesis in mammalian and human cells induced by ionizing radiation with varying LET. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 1997, vol. 37, no. 4, pp. 539-548. (In Russian).

4. Valko M., Leibfritz D., Moncol J., Cronin M.T.D., Mazur M.,Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol., 2007, vol. 39, pp. 44-84.

5. Sokolovsky V.V. Tiol-disufide system in organism reaction to environmental factors. Sankt-Peterburg, Nauka, 2008. 112 p. (In Russian).

6. Pruijn F.B., Haenen G.R.M.M., Bast A. Integray between vitamin E, glutathione and dihydrolipoic acid in protection against lipid peroxidation. Fat. Sci. Technol., 1991, vol. 93, no. 6, pp. 216-221.

7. Pastore A., Federici G., Bertini E., Piemonte F. Analysis of glutathione: implication in redox and detoxification. Clin. Chim. Acta, 2003, vol. 333, no.1, pp. 19-39.

8. Selivanova E.I., Zamulaeva I.A., Saenko A.S. Influence of chronic irradiation on the distribution of blood lymphocyte subpopulation among professionals of the atomic industry. Radiation Biology. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 2014, vol. 54, no. 2, pp. 153-161. (In Russian).

9. McNeil T.L., Beck L.Y. Fluorometric estimation of GSH-OPT. Anal. Biochem. 1968, vol. 22, pp. 431-441.

10. Ivanenko G.F., Suskov I.I., Burlakova E.B. Possible relationships between plasma glutathione levels and cytogenetic indices in peripheral blood lymphocytes of children exposed to low doses of radiation. Izvestiya RAN. Seriya biologicheskaya – Bulletin RAS. Series of Biological, 2004, no. 4, pp. 410-415. (In Russian).

11. Hansen L.G., Warwick W.G. A fluorometric micromethod for serum vitamins A and E. Tech. Bull. Regist. Med. Technol., 1969, vol. 39, no. 3, pp. 70-73.

12. Bandazhevsij Yu.I. Radiocaesium and intrauterine fetal development. Minsk, Belrad, 2001. 54 p. (In Russian).

13. Tarasenko L.V., Bezdrobnaja L.K., Buhal A.V., Cyganjuk T.V., Romanova E.P., Trishin V.V. Cytogenetic effects in blood lymphocytes of Slavutich residents having no occupational contact with ionizing radiation. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology 2004, vol. 44, no. 6, pp. 632-636. (In Russian).

14. Zhizhina G.P., Zavarykina T.M., Mil' E.M., Burlakova E.B. The effects of low doses of low-level γ-radiation and phenozan injection on structural characteristics of mice spleen DNK. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 2007, vol. 47, no. 4, pp. 414-422. (In Russian).

15. Chatterjee F. Reduced Glutathione: a radioprotector or a modulator of DNK-repair activity? Nutrients, 2013, vol. 5, pp. 525-542.

16. Kemp M., Go Y.M., Jones D.P. Nonequilibrium thermodynamics of thiol/disulfide systems: a perspective on redox systems biology. Free Radic. Biol. Med., 2008, vol. 44, pp. 921-937.

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