Authors

Khvostunov I.K. – Head of Lab., D. Sc., Biol., Medical Radiological Research Center of the Russian Ministry of Health, Obninsk. Contacts: 4 Korolyov str., Obninsk, Kaluga region, Russia, 249036. Tel: (48439) 9-73-92; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Pyatenko V.S. – Leading Researcher, C. Sc., Biol., Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.
Shepel N.N. – Senior Researcher, C. Sc., Biol., Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.
Korovchuk O.N. – Researcher, Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.
Golub E.V. – Leading Researcher, D. Sc., Biol., Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.
Zhironkina A.S. – Researcher, Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.
Khvostunova T.I. – Researcher, Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.
Lychagin A.A. – Head of lab., C. Sc., Phys.-Math., Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.

Abstract

Relationship between radiation doses and frequency of chromosome aberrations in irradiated human blood lymphocytes and Chinese hamster ovary (CHO) was investigated. Chromosome aberrations were analyzed in cells collected at first mitosis following exposure in vitro to gamma-rays of ⁶⁰Co, accelerated 73 MeV protons and 14 MeV neutrons. After irradiation with gamma-rays of ⁶⁰Co and 14 MeV neutrons survival of CHO cells was estimated by the colony formation assay. Regression coefficients of dose response curves and corresponding values of relative biological effectiveness were estimated by statistical analysis using obtained data in the form of chromosome aberration frequency and cell survival. Investigated regularities of chromosome aberrations induction in human blood lymphocytes can be used not only for cytogenetic bioldosimetry but also for estimation of individual radiation sensitivity of lymphocytes and investigation of radiation-induced chromosomal instability in humane genome.

Key words
Cancer, recurrence, adjuvant radiation therapy, neutron therapy.

References

1. Elisova T.V. Stabil'nye i nestabil'nye aberratsii khromosom u cheloveka i drugikh mlekopitaiushchikh v sviazi s voprosami biologicheskoi dozimetrii Radiatsionnaia biologiia. Radioekologiia. 2008. T. 48, № 1. pp. 14-27.
2. Okada Sh. Radiatsionnaia biokhimiia kletki /Per. s angl. M.: Mir, 1974. 407 p.
3. Ul'ianenko S.E., Lychagin A.A., Koriakin S.N., Khvostunov I.K. i dr. Radiobiologicheskie otsenki impul'snogo protonnogo izlucheniia uskoritelia I-100 Meditsinskaia fizika. 2009. T. 44, № 4. pp. 8-16.
4. Khvostunov I.K., Kursova L.V., Shepel' N.N. i dr. Otsenka tselesoobraznosti primeneniia biologicheskoi dozimetrii na osnove analiza khromosomnykh aberratsii v limfotsitakh krovi bol'nykh rakom legkogo pri terapevticheskom fraktsionirovannom -obluchenii Radiatsionnaia biologiia. Radioekologiia. 2012. T. 52, № 5. pp. 467-480.
5. Anderson R.M., Stevens D.L., Goodhead D.T. M-FISH analysis shows that complex chromosome aberrations induced by a-particle tracks are cumulative products of localized rearrangements PNAS. 2002. V. 99, N 19. pp. 12167-12172.
6. Chudoba I., Plesch A., Lörch T. et al. High resolution multicolor-banding: a new technique for refined FISH analysis of human chromosomes Cytogenet. Cell Genet. 1999. V. 84, N 3-4. pp. 156-160.
7. Cytogenetic analysis for radiation dose assessment: A Manual - (Technical Reports Series/IAEA; № 405) /International Atomic Energy Agency. Vienna: IAEA, 2001. 127 p.
8. Edwards A.A., Lloyd D.C., Purrott R.J. Radiation induced chromosome aberrations and the Poisson distribution Radiat. Environm. Biophys. 1979. V. 16, N 2. pp. 89-100.
9. Kligerman A.D., Halperin E.C., Erexson G.L. et al. A cytogenetic comparison of the responses of mouse and human peripheral blood lymphocytes to 60Co gamma radiation Radiation Research. 1988. V. 115, N 2. pp. 334-346.
10. Leuthold G., Brenner H. Critical analysis of the ICRU 60 Proposal for neutron radiation and a possible solution Radiation Protection Dosimetry. 1994. V. 54, N 3/4. pp. 217-220.
11. Lloyd D.C., Purrott R.J., Dolphin G.W. et al. The relationship between chromosome aberrations and low LET radiation dose to human lymphocytes International Journal of Radiation Biology. 1975. V. 28, N 1. pp. 75-90.
12. Lucas J.N., Awa A., Stranme T., Gray M., Littlefield G. Rapid translocation frequency analysis decades after exposure to ionizing radiation International Journal of Radiation Biology. 1992. V. 62, N 1. pp. 53-63.

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