Radiation decontamination of dill fruits (Anethi graveolentis fructus)

«Radiation and Risk», 2025, vol. 34, No. 1, pp.106-114

DOI: 10.21870/0131-3878-2025-34-1-106-114

Authors

Kharlamov V.A. – Sen. Researcher, C. Sc., Biol. Contacts: 1, Kievskoe shosse, Obninsk, Kaluga region, Russia, 249035. Tel.: 8(910) 518-81-17; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Frolova N.A. – Researcher, C. Sc., Biol.
Vasileva N.A. – Researcher, C. Sc., Biol.
Gubina O.A. – Researcher
Krylenkin D.V. – Researcher
Dikarev A.V. – Researcher, C. Sc., Biol.
Chizh T.V. – Jr. Researcher, C. Sc., Biol.
Snegirev A.S. – Jr. Researcher, C. Sc., Biol. NRC “Kurchatov Institute” – RIRAE.
Russian Institute of Radiology and Agroecology of National Research Centre “Kurchatov Institute”, Obninsk

Abstract

The aim of the work was to investigate the effects of ionizing radiation on microbial contamination and the chemical composition. For analysis infrared spectra of dill fruits have been used. The object of the study was dill fruits (Anethi graveolentis fructus), used both as food and medicinal plant material. The effects of gamma and electron radiation doses on microbiological properties of the plant material were investigated. The study was carried out in accordance according to the methodological guidelines of the General Pharmacopoeia Monograph “Microbiological Purity (the 15th edition of the State Pharmacopeia of the Russian Federation). The optimal radiation dose to dill weeds decontamination was found to be 9 kGy of gamma radiation and 5 kGy of electron radiation. Infrared spectra of dill weeds were used as the control, unirradiated and those exposed to electron beams and gamma rays. No changes in peak maxima positions were observed. It means that the structure of chemical bonds remains intact. Thus, new experimental data were obtained, providing a more detailed understanding of the biocidal effects of various types of ionizing radiation on the studied plant material, as well as the impact of radiation treatment on its chemical composition. The results of this study have practical significance, as they aim to improve the quality and safety of herbal medicinal products. Radiation treatment effectively ensures the microbiological purity of products, extending their shelf life and simplifying storage, thereby making the products more accessible to consumers.

Key words
herbal medicinal raw material, Anethi graveolentis fructus, radiation decontamination, gamma radiation, electron radiation, total aerobic microbial count, total yeast and mold count, infrared spectra, radiobiology, public health.

References

1. Ovuru K.F., Izah S.C., Yasmin H., Enerijiofi K.E., Das M., Ogwu M.C. Microbial contaminants of herbal remedies: health risks and sustainable quality control strategies. Herbal Medicine Phytochemistry: Applica-tions and Trends. Cham, Springer Int. Publ, 2024, pp. 1571-1600.

2. Gallo M., Ferrara L., Calogero A., Montesano D., Naviglio D. Relationships between food and diseases: what to know to ensure food safety. Food Res. Int., 2020, vol. 137, pp. 109414. DOI: 10.1016/j.foodres.2020.109414.

3. Adie G.U. Chemical and microbial contamination of herbal remedies and their potential health implications: a review. Proc. Int. Acad. Ecol. Environ. Sci., 2022, vol. 12, no. 2, pp. 54-73.

4. Efimochkina N.R., Sedova I.B., Sheveleva S.A., Tutelyan V.A. Toxigenic properties of mycotoxin-producing fungi. Vestnik Tomskogo gosudarstvennogo universiteta. Biologiya – Tomsk State University Journal of Biology, 2019, no. 45, pp. 6-33. (In Russian).

5. Rajanna S.K.S., Jayanna N.K.K., Bongale M.M., Srinivasa C. Role of foodborne pathogens and microor-ganisms in food safety. Global Food Safety. New York, Apple Academic Press, 2023, pp. 57-91.

6. Kannan P., Ruban S.W., Quintoil M.N. Foodborne pathogens and nanoparticles as a tool for quality assur-ance and intervention of foodborne pathogens. Environmental Technology and Engineering Techniques. New York, Apple Academic Press, 2020, pp. 133-153.

7. Koz’min G.V., Geras’kin S.A., Sanzharova N.I. Radiation technologies in agriculture and food industry. Obninsk, RIRAE, 2015. 400 p. (In Russian).

8. Wholesomeness of irradiated food. Report of a Joint FAO/IAEA/WHO Expert Committee. WHO Technical Report Series No. 659. Geneva, WHO, 1981.

9. Code of practice for radiation processing of food. Codex Alimentarius Commission. CAC/RCP 19-1979, Rev.2-2003. Rome, FAO/WHO, 2003.

10. Wang Y., Zhou A., Yu B., Sun X. Recent advances in non-contact food decontamination technologies for removing mycotoxins and fungal contaminants. Foods, 2024, vol. 13, no. 14, pp. 2244. DOI: 10.3390/foods13142244.

11. Khawory M.H., Sain A.A., Rosli M.A.A., Ishak M.S., Noordin M.I., Wahab H.A. Effects of gamma radiation treatment on three different medicinal plants: microbial limit test, total phenolic content, in vitro cytotoxicity effect and antioxidant assay. Appl. Radiat. Isot., 2020, vol. 157, p. 109013. DOI: 10.1016/j.apradiso.2019.109013.

12. Rahmati E., Khoshtaghaza M.H., Banakar A., Ebadi M.T. Decontamination technologies for medicinal and aromatic plants: a review. Food Sci. Nutr., 2022, vol. 10, no. 3, pp. 784-799.

13. Paniagua-Zambrana N.Y., Bussmann R.W., Kikvidze Z., Khojimatov O.K. Anethum graveolens L. Apiaceae. Ethnobotany of the Mountain Regions of Eastern Europe. Ethnobotany of Mountain Regions. Cham, Springer, 2024.

14. Sadeghi M., Kabiri S., Amerizadeh A., Heshmat-Ghahdarijani K., Masoumi G., Teimouri-Jervekani Z., Amirpour A. Anethum graveolens L. (Dill) effect on human lipid profile: an updated systematic Review. Curr. Probl. Cardiol., 2022, vol. 47, no. 11, pp. 101072. DOI: 10.1016/j.cpcardiol.2021.101072.

15. Junaedi E.C., Lestari K., Muchtaridi M. Infrared spectroscopy technique for quantification of compounds in plant-based medicine and supplement. J. Adv. Pharm. Technol. Res., 2021, vol. 12, no. 1, pp. 1-7. DOI: 10.4103/japtr.JAPTR_96_20.

16. Sanyal B., Ahn J.J., Maeng J.H., Kyung H.K., Lim H.K., Sharma A., Kwon J.H. An improved approach to identify irradiated spices using electronic nose, FTIR, and EPR spectroscopy. J. Food Sci., 2014, vol. 79, no. 9, pp. C1656-C1664.

17. Polesskaya O.G. Plant cell and reactive oxygen species: a textbook. Ed.: I.P. Ermakov. Moscow, KDU, 2007. 140 p. (In Russian).

18. Jadhav V.K., Pawar S.S. FTIR spectroscopic analysis of Anethum graveolens L. leaves extract. J. Indian Bot. Soc., 2024, vol. 104, no. 2, pp. 101-107.

19. Naidu M.M., Vedashree M., Satapathy P., Khanum H., Ramsamy R., Hebbar H.U. Effect of drying methods on the quality characteristics of dill (Anethum graveolens) greens. Food Chem., 2016, vol. 192, pp. 849-856.

Full-text article (in Russian)