The effect of chronic low-dose gama-irradiation on the behavior of mice and their offspring

«Radiation and Risk», 2025, vol. 34, No. 1, pp.46-54

DOI: 10.21870/0131-3878-2025-34-1-46-54

Authors

Sorokina S.S. – Sen. Researcher, C. Sc., Biol.
Popova N.R. – Head of Lab, C. Sc., Biol. ITEB RAS. Contacts: 3 Institutskaya str, Pushchino, Moscow region, Russia, 142290. Tel.: +7 (4967)73-94-31; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Institute of Theoretical and Experimental Biophysics of Russian Academy of Sciences, Pushchino

Abstract

In the last decade, there has been scientific interest in the potential non-cancer effects of low (<0.1 Gy) and moderate (0.1-0.5 Gy) doses of ionizing radiation. In particular, special attention has been paid to the cognitive and neurodegenerative effects of low-dose radiation therapy for tumors and low doses of gama- and X-ray radiation after medical procedures. The aim of the work was to study the effect of chronic low-dose gama-irradiation on the behavior of mice and their offspring in the acute period after the exposure. Male SHK mice (F0, 4 weeks) were totally irradiated with the use of ⁶⁰Co unit (dose rate 3.9 cGy/day, IBC RAS, Pushchino) until a total dose of 0.1 Gy was accumulated. In 2 weeks after irradiation, the males were crossed with non-irradiated females to obtain F1 offspring. The general activity and anxiety level, spatial learning, long-term and short-term hippocampus-dependent memory were assessed in irradiated males 1 day after exposure, and in offspring – upon reaching the age of 4 weeks. The obtained results revealed the absence of anxiety in both F0 and F1 groups of mice, while animals subjected to long-term gama-irradiation at a dose of 0.1 Gy were characterized by higher locomotor activity and a reliable increase in the total time spent in the central zone of the open field test. In addition, the irradiated males demonstrated the fastest dynamics of skill acquisition in the Barnes maze with preservation of the memory trace, indicating the absence of disturbances in spatial learning and long-term hippocampus-dependent memory. At the same time, irradiated animals showed a disturbance of episodic memory in the novel object recognition test. Unchanged behavioral pattern and cognitive abilities compared to control animals were observed in the F1 group of mice, indicating the absence of genetic instability in the offspring of irradiated males for the studied behavioral parameters.

Key words
gama-radiation, low-dose radiation therapy, low doses, behavior, cognitive deficit, hippocampus, episodic memory, mice, radiobiology.

References

1. Hall P., Adami H.-O., Trichopoulos D., Pedersen N.L., Lagiou P., Ekbom A., Ingvar M., Lundell M., Granath F. Effect of low doses of ionising radiation in infancy on cognitive function in adulthood: Swedish population-based cohort study. BMJ, 2004, vol. 328, no. 7430, pp. 19. DOI: 10.1136/bmj.328.7430.19.

2. Armstrong G.T., Jain N., Liu W., Merchant T.E., Stovall M., Srivastava D.K., Gurney J.G., Packer R.J., Robison L.L., Krull K.R. Region-specific radiotherapy and neuropsychological outcomes in adult survivors of childhood CNS malignancies. Neuro Oncol., 2010, vol. 12, no. 11, pp. 1173-1186.

3. Chen J.-H., Yen Y.-C., Liu S.-H., Lee F.-P., Lin K.-C., Lai M.-T., Wu C.-C., Chen T.-M., Yuan S.-P., Chang C.-L., Wu S.-Y. Dementia risk in irradiated patients with head and neck Cancer. Medicine (Baltimore), 2015, vol. 94, no. 45, pp. e1983. DOI: 10.1097/MD.0000000000001983.

4. Stuart F.A., Akleyev A.V., Hauer-Jensen M., Hendry J.H., Kleimen N.J., McVittie T.J., Ailman B.M., Edgar A.B., Mabuchi K., Muirhead K.R., Shore R.I., Wallace W.H. Proceedings of the ICRP. Eds.: A.V. Akleyev, M.F. Kiselev. Chelyabinsk, Book, 2012. 384 p. (In Russian).

5. Sorokina S.S., Malkov A.E., Shubina L.V., Zaichkina S.I., Pikalov V.A. Low dose of carbon ion irradiat

ion induces early delayed cognitive impairments in mice. Radiat. Environ. Biophys., 2021. vol. 60, no. 1, pp. 61-71.

6. Lowe X.R., Bhattacharya S., Marchetti F., Wyrobek A.J. Early brain response to low-dose radiation expo-sure involves molecular networks and pathways associated with cognitive functions, advanced aging and Alzheimer’s disease. Radiat. Res., 2009. vol. 171, no. 1, pp. 53-65.

7. Veeraraghavan J., Natarajan M., Herman T.S., Aravindan N. Low-dose -radiation-induced oxidative stress response in mouse brain and gut: regulation by NFkB-MnSOD cross-signaling. Mutat. Res., 2011, vol. 718, no. 1-2, pp. 44-55.

8. Rodina A.V., Semochkina Y.P., Vysotskaya O.V., Romantsova A.N., Strepetov A.N., Moskaleva E.Y. Low dose gamma irradiation pretreatment modulates the sensitivity of CNS to subsequent mixed gamma and neu-tron irradiation of the mouse head. Int. J. Radiat. Biol., 2021, vol. 97, no. 7, pp. 926-942.

9. Bellone J., Gifford P., Nishiyama N., Hartman R.E., Mao X.W. Long-term effects of simulated microgravity and/or chronic exposure to low-dose gamma radiation on behavior and blood-brain barrier integrity. NPJ Microgravity, 2016, vol. 2, pp. 16019. DOI: 10.1038/npjmgrav.2016.19.

10. Ung M.-C. The long-term effects of low dose ionizing radiation on the adult mouse brain and consequences for behavior: dissertation. München, 2021. 160 p. Available at: https://media-tum.ub.tum.de/doc/1575638/1575638.pdf (Accessed 07.12.2024).

11. York J.M., Blevins N.A., Meling D.D., Peterlin M.B., Gridley D.S., Cengel K.A., Freund G.G. The biobe-havioral and neuroimmune impact of low-dose ionizing radiation. Brain Behav. Immun., 2012. vol. 26, no. 2, pp. 218-227.

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