Antitumor efficiency of photodynamic therapy of Ehrlich carcinoma mice with the photosensitizer Photoran E6

«Radiation and Risk», 2022, vol. 31, No. 2, pp.162-173

DOI: 10.21870/0131-3878-2022-31-2-162-173

Authors

Abramova O.B. – Head of Lab., C. Sc., Med. Contacts: 4 Korolyov str., Obninsk, Kaluga region, Russia, 249035. Tel.: + 7 910 510 08 62; е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Kaplan M.A. – Head of Dep., MD, Prof.
Yuzhakov V.V. – Head of Lab., C. Sc., Med.
Drozhzhina V.V. – Researcher
Bandurko L.N. – Lead. Researcher, C. Sc., Med.
Yakovleva N.D. – Lead. Researcher, C. Sc., Biol.
Churikova T.P. – Research Assistant
Kozlovtseva E.A. – Research Assistant
Ivanov S.A. – Director, MD, Prof. A. Tsyb MRRC.
Кaprin A.D. – General Director, Academician of RAS, MD, Prof. NMRRC.
A. Tsyb MRRC, Obninsk
¹ NMRRC Russian Ministry of Health, Moscow

Abstract

Today, photodynamic therapy (PDT) is one of the most promising methods for the complete eradication of a number of superficial solid malignant neoplasms. In this work, we studied the effectiveness of PDT on Ehrlich carcinoma of mice with the injections of a new photosensitizer (PS) Photoran E6. To determine the optimal druglight interval, from the time of PS injection to laser irradiation, we studied the distribution kinetics of Photoran E6 in tumors and healthy tissues surrounding them. In assessing the response of the inhibitory effect of Ehrlich carcinoma to PDT, the following indicators were used: inhibition of tumor growth (ITG, %), complete regression (CR, %), absolute growth rates of tumors (K) in mice with continued growth of neoplasia, and an increase in life expectancy (ILE, %) of individuals in the experimental groups compared with the control, according to the criterion of curing animals: the absence of signs of tumor recurrence within 90 days after therapy. The therapeutic potential of PDT for tumor cell devitalization was determined by histological examination of the areas of exposure to laser radiation at 21d after treatment. The results showed that Photoran E6 has a high antitumor activity for PDT of solid Ehrlich carcinoma. Conducting PDT in the optimal time after the injection of PS with certain parameters of laser exposure allows to achieve the maximum inhibitory effect on malignant neoplasms. A pathomorphological study in the areas of photodynamic exposure of the surviving tumor cells was not found.

Key words
photodynamic therapy, photosensitizer, Ehrlich carcinoma, selective photosensitizer accumulation, contrast index, tumor regression, pathomorphology, tumor cell devitalization, Photoran E6.

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