Development of fluorescence diagnosis and photodynamic therapy in A. Tsyb Medical Radiological Research Centre

«Radiation and Risk», 2022, vol. 31, No. 2, pp.139-150

DOI: 10.21870/0131-3878-2022-31-2-139-150

Authors

Popovkina O.E. – Head Dep., C. Sc., Med.
Kapinus V.N. – Sen. Researcher, C. Sc., Med. Contacts: 4 Korolyov str., Obninsk, Kaluga region, Russia, 249035. Tel.: (484) 399-32-36; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Yaroslavtseva-Isaeva E.V. – Lead. Researcher, C. Sc., Med.
Spichenkova I.S. – Physician, C. Sc., Med.
Borgul O.V. – Physician, C. Sc., Med.
Ivanov S.A. – Director, MD, Prof. of RAS. A. Tsyb MRRC.
Romanko Yu.S. – Sen. Researcher, MD, Prof. Sechenovskiy University.
Kaprin A.D. – General Director, Academician of RAS, MD, Prof. NMRRC.
¹ A. Tsyb MRRC, Obninsk
² NMRRC, Moscow
² Peoples’ Friendship University of Russia, RUDN University, Moscow
² I.M. Sechenov First Moscow State Medical University, Moscow

Abstract

Clinical application of photodynamic therapy (PDT) and fluorescence diagnosis is being extended in Russia. A. Tsyb MRRC scientists headed by Professor M. Kaplan have conducted experimental studies of the photosensitizers (PS): Photochem, Photosens, Photolon, Photoditazin, Radachlorin, Photoran, belonged to different classes, estimated their therapeutic effectiveness for malignant tumors of different histologic type. From the study of basic mechanisms of photochemical reactions with chlorine photosensitizers it became evident that the photosensitizers destroyed microciculatory bed, inhibited proliferation and tumor cells functional activity, induced apoptosis and necrosis at early stages of a tumor development. Effects of PS dosage and dose of light to be delivered to a target on a treatment outcome were studied. The results of experimental studies were used to support clinical application of focal, interstitial, systemic techniques with different PS as individual treatment modalities or as a modality combined with radiation therapy, surgery, chemotherapy, electrochemical lysis. Optimal PS and light exposure doses were determined, indications for the use of optimal PS and light doses for treatment of malignant neoplasms of the skin and the mucous membranes were formulated. Due to the use of high-tech effective treatment modalities developed in the Department of Fluorescence Diagnosis and Photodynamic Therapy of A. Tsyb Medical Radiological Research Centre – the branch of the Medical Research Radiological Centre thousands of patients have been cured.

Key words
photodynamic therapy, intraoperative photodynamic therapy, systemic photodynamic therapy, photosensitizer, photohem, photosens, photolon, photoran, photoditazine, fluorescence diagnosis, skin neoplasms, malignant neoplasms, cardiomarkers.

References

1. Agostinis P., Berg K., Cengel K.A., Foster T.H., Girotti A.W., Gollnick S.O., Hahn S.M., Hamblin M.R., Juzeniene A., Kessel D., Korbelik M., Moan J., Mroz P., Nowis D., Piette J., Wilson B.C., Golab J. Photodynamic therapy of cancer: an update. CA Cancer J. Clin., 2011, vol. 61, no. 4, pp. 250-281.

2. Dolmans D.E., Fukumura D., Jain R.K. Photodynamic therapy for cancer. Nat. Rev. Cancer, 2003, vol. 3, no. 5, pp. 380-387.

3. Gamayunov S.V., Korchagina K.S. Local therapeutic methods for basal cell carcinoma. Effektivnaya farmakoterapiya – Effective Pharmacotherapy, 2016, vol. 39, pp. 74-83. (In Russian).

4. Tsyb A.F., Kaplan M.A., Romanko Yu.S., Popuchiyev V.V. The effect of photodynamic therapy with Photoditazine on the morphofunctional characteristics of M-1 sarcoma. Byulleten eksperimentalnoy biologii i meditsiny – Bulletin of Experimental Biology and Medicine, 2004, no. 12, pp. 658-664. (In Russian).

5. Kaplan M.A., Ponomarev G.V., Baum R.F., Romanko Yu.S., Mardynskaya V.P., Malygina A.I. Study of the specific photodynamic activity of Photoditazine in photodynamic therapy in experimental tumor-bearing animals. Rossiyskiy bioterapevticheskiy zhurnal – Russian Biotherapeutic Journal, 2003, vol. 2, no. 4, pp. 23-30. (In Russian).

6. Petrov P.T. Experimental studies of Photolon as a means for photodynamic diagnostics and therapy of ma-lignant neoplasms. Man and medicine: Proceedings of the X Russian National Congress, Moscow, April 7-11, 2003. Moscow, 2003, pp. 20-25. (In Russian).

7. Petrov P.T. New aspects of clinical application of PDT with Photolon (Fotolon). 12 Intern. Congress of the European Medical Laser Association in conjunction with the World Association of Laser Therapy: book of abstracts, Prague, 20-22 September, 2007. Prague, 2007, p. 18. (In Russian).

8. Hwang H.S., Shin H., Han J., Na K. Combination of photodynamic therapy (PDT) and anti-tumor immunity in cancer therapy. J. Pharm. Investig., 2018, vol. 48, no. 2, pp. 143-151.

9. Maeding N., Verwanger T., Krammer B. Boosting tumor-specific immunity using PDT. Cancers, 2016, vol. 8, no. 10, pp. 91. DOI: 10.3390/cancers8100091.

10. Wachowska M., Muchowicz A., Demkow U. Immunological aspects of antitumor photodynamic therapy out-come. Cent. Eur. J. Immunol., 2015, vol. 40, no. 4, pp. 481-485. DOI: 10.5114/ceji.2015.56974.

11. Naidoo C., Kruger C.A., Abrahamse H. Photodynamic therapy for metastatic melanoma treatment: a review. Technol. Cancer Res. Treat., 2018, vol. 17, pp. 1-15. DOI: 10.1177/1533033818791795.

12. Beltrán Hernández I, Yu Y., Ossendorp F, Korbelik M., Oliveira S. Preclinical and clinical evidence of immune responses triggered in oncologic photodynamic therapy: clinical recommendations. J. Clin. Med., 2020, vol. 9, no. 2, pp. 333. DOI: 10.3390/jcm9020333.

13. Jain M., Zellweger M., Wagnières G., van den Bergh H., Cook S., Giraud M.-N. Photodynamic therapy for the treatment of atherosclerotic plaque: lost in translation? Cardiovasc. Ther., 2017, vol. 35, no. 2, pp. 1-14. DOI: 10.1111/1755-5922.12238.

14. Han X., Kou J., Zheng Y., Liu Z., Jiang Y., Gao Z., Cong L., Yang L. ROS generated by upconversion nanoparticle-mediated photodynamic therapy induces autophagy via PI3K/AKT/Mtor signaling pathway in M1 peritoneal. Cell Physiol. Biochem., 2019, vol. 52, no. 6, pp. 1325-1338. DOI: 10.33594/000000093.

15. Efremova Yu.E., Soboleva G.N., Andreyeva E.R., Karpov Yu.A., Tararak E.M. Photodynamic therapy in cardiovascular pathology. Atmosfera. Novosti kardiologii – Atmosphere. Cardiology News, 2010, no. 2-3, pp. 15-18. (In Russian).

16. Vozovikov I.N., Andreeva E.R., Yanzen E.S., Kuzmin S.G., Tararak E.M. Possibilities of using photody-namic therapy for the treatment and prevention of cardiovascular diseases. Kardiologicheskiy vestnik – Cardiological Bulletin, 2006, vol. 13, no. 1, pp. 52-55. (In Russian).

17. Waksman R., McEwan P.E., Moore T.I., Pakala R., Kolodgie F.D., Hellinga D.G., Seabron R.C., Rychnovsky S.J., Vasek J., Scott R.W., Virmani R. Photo Point photodynamic therapy promotes stabilization of atherosclerotic plaques and inhibits plaque progression. J. Am. Coll. Cardiol., 2008, vol. 52, no. 12, pp. 1024-32. DOI: 10.1016/j.jacc.2008.06.023.

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