Study of efficiency of photodynamic therapy of Earlich carcinoma mice with intravenous introduction of photosensibilizer of liposomal boronated chlorine e6

«Radiation and Risk», 2019, vol. 28, No. 4, pp.96-107

DOI: 10.21870/0131-3878-2019-28-4-96-107


Burmistrova N.V. – Head of Lab., C. Sc., Med.
Drozhzhina V.V. – Researcher. Contacts: 4 Korolyov str., Obninsk, Kaluga region, Russia, 249035. Tel.: + 7 (903) 524-53-13; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Kaplan M.A. – Head of Dep., MD, Prof.
Yuzhakov V.V. – Head of Lab., C. Sc., Med.
Bandurko L.N. – Lead. Researcher, C. Sc., Med.
Tsyganova M.G. – Researcher
Ivanov S.A. – Director, MD, Prof.
Arkhipova L.M. – Research Assistant
Abramova O.B. – Researcher

A. Tsyb MRRC, Obninsk.


The article is devoted to the study of the effectiveness of photodynamic therapy (PDT) with the use of new Russian photosensitizer (PS) of liposomal boronated chlorin e6 after its intravenous injection. As an experimental model of the tumor, Ehrlich carcinoma of mice was studied. The aim of the study was to study the effectiveness of PDT with the use of liposomal boronated chlorin e6 as PS. To determine the optimal time for laser irradiation, the kinetics of the distribution of PS in tumor and healthy thigh tissues was studied. The effectiveness of therapy was assessed by the number of animals with complete tumor regression (CR%), by inhibition of tumor growth (TGI%) and by the absolute growth rate (K) in animals with continued tumor growth compared with the control. Control mice were tumor carriers without exposure. The therapeutic potential of PDT for the devitalization of tumor cells was verified by histological studies. It was found that after PDT, which was carried out at the optimal time after the injection of PS, complete tumor regression was observed in 100% of the animals before 21 days of the study at a dose of PS of 1.25 mg/kg, at energy density of 150 J/cm2 and a power density of 0.51 W/cm2 laser radiation. When microscopic examination of tissues in the laser exposure zone, only one animal with visually regressed Ehrlich carcinoma was found to have a relapse of the neoplasm..

Key words
photodynamic therapy, Ehrlich carcinoma, mice, liposomal boronated chlorin e6, photosensitizer accumulation dynamics, contrast index, laser, tumor regression, pathomorphology.


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