Radiomodifying effects of calcium salts on laboratory mice

«Radiation and Risk», 2019, vol. 28, No. 2, pp.51-60

DOI: 10.21870/0131-3878-2019-28-2-51-60


Ivanov V.L. – Senior Researcher, C. Sc., Vet. Contacts: 4 Korolev str., Obninsk, Kaluga region, Russia, 249036. Tel: (484) 399-71-38; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Panfilova V.V. – Researcher, C. Sc., Biol.
Chibisova O.F. – Researcher.

A. Tsyb MRRC, Obninsk, Russia


Study of substances that could protect against radiation effects, lethality and genotoxicity, started in the beginning of the nuclear era. At the present time research and development of new more effective compounds, reducing radiation effects on humans and biota, is continuing. Known radioprotective agents can be divided into radioprotectors including radiomitigators, adaptogens and adsorbents. Adaptogens enhance resistance of a body to radiation, they activate antioxidant, reparation and other protective systems. The most part of adaptogens have low toxicity, due to their properties to activate regulatory systems the adaptogens also activate the protective response to radiation. It is known that calcium-containing salts protect a body during external acute or chronic exposure to radiation, and internal exposure to incorporated radioactive substances. The authors wrote that exposure to radiation causes reduction of calcium level in the blood and together with that calcium level in tissues enhanced sharply (by 15 times). This can be due to disturbance of calcium metabolism in a body. The paper aims to study if calcium salts of carbonic and gluconic acids activate radioresistance of mice exposed to gamma-radiation. The diet of white outbred mice (females) was fortified with calcium salts (0.1 g/animal), or calcium salts and vitamins A and D2. The mice consumed the food fortified with calcium salt 3-7 days before exposure to radiation (dose – 8 Gy, dose rate – 2 Gy/min) and 6-10 days after exposure. 30-Day survival of animals fed food fortified with calcium salt enhanced by 80%, whilst the survival of mice in the control group consumed food free of calcium salts after exposure to 8 Gy was 31.7%. Adding 10IU of vitamin A and 1IU of vitamin D2 to food allowed increasing the survival by 10.5 and 15.0% more, and total survival was 90.5 and 95.0%. It is interesting, that survival of mice begun the consumption of food fortified with calcium carbonate in 4 days after irradiation was 80% higher than in miceconsumed food without additives. All indications are that calcium salt added to mice diet acts as adoptogen and has beneficial effect on survival of irradiated animals.

Key words
Gamma-radiation, white outbred mice, adaptogens, calcium carbonate, calcium gluconate, vitamin A (retinol), vitamin D2 (ergocalciferol), feed allowances, radioresistance, animal body mass.


1. Saksonov P.P., Shashkov V.S., Sergeev P.V. Radiation pharmacology. Moscow, Medicine, 1976. 256 p. (In Russian).

2. Baranov A.E., Rozhdestvenskiy L.M. Analytical review of schemes of treatment of acute radiation sickness, used in experiment and clinic. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 2008, vol. 48, no. 3, pp. 287-302. (In Russian).

3. Yarmonenko S.P., Vaynson A.A. Radiobiology of humans and animals. Moscow, High school, 2004. 549 p. (In Russian).

4. Yarmonenko S.P. Antiradiation protection of the body. Moscow, Atomizdat, 1969. 264 p. (In Russian).

5. Brekhman I.I. Eleutherococcus. Leningrad, Science, 1968. (In Russian).

6. Knizhnikov V.A. Calcium and fluoride. Radiation-genetic aspects. Moscow, Atomizdat, 1975. 200 p. (In Russian).

7. Orlov A.F. Thyrocalcitonin – gipocalziemicescoe hormone of the thyroid gland. Problemy endokrinologii – Problems of Endocrinology, 1969, pp. 109-117. (In Russian).

8. Afonsky S.I. Biochemistry of animals. Moscow, High school, 1964. 630 p. (In Russian).

9. Comar C.L., Wasserman R.H. Mineral metabolism. Chapter 23. Strontium. New York, Academic press, 1964, pp. 54-57.

10. Kazbekova D.A., Kalistratova V.S., Kulyamin V.A. Radioprotective effect of calcium administered with food, with the defeat of the oxide of tritium. Radiobiologiya – Radiobiology, 1979, vol. XIX, no. 3, pp. 402- 407. (In Russian).

11. Belousova O.I., Grozdovskaya V.A., Knizhnikov V.A. The influence of strontium-90 on hematopoiesis in rats under conditions of increased intake of fluoride and calcium with a diet. Radiobiologiya – Radiobiology, 1967, no. 2, pp. 297-300. (In Russian).

12. Knizhnikov V.A. Reducing the accumulation of strontium-90 in the body by adding minerals to water and food. Collection of abstracts on radiation medicine. Moscow, Medgiz, 1962. 158 p. (In Russian).

13. Knizhnikov V.A., Grozdovskaya V.A. Influence of calcium and fluorine level in the diet on the resistance of animals to the combined damage of external gamma radiation and incorporated strontium-90. Voprosy pitaniya – Nutrition Questions, 1968, no. 4, pp. 24-30. (In Russian).

14. Perepelkin S.R. Protective effect of food and vitamins in radiation lesions of the body. Moscow, Medicine, 1965. 81 р. (In Russian).

15. Zhavoronkov L.P. Fundamentals of applied biomedical statistics: a Methodological guide. Obninsk, MRRC, 2012. 60 p. (In Russian).

16. Breslow N.E., Day N.E. Statistical methods in cancer research. Vol. I: The analysis of case-control studies. IARC Scientific Publication No. 32. Lyon, IARC, 1980. 338 p.

17. Grozdov S.P. Radiation characteristics of calcium metabolism in tissues of rats and their relationship with the state of the exchange of sodium and phosphorus. Radiobiologiya – Radiobiology, 1974, vol. XIV, no. 3, pp. 342-347. (In Russian).

18. Kuznetsova N.E. Restorative processes in radiation damage. Moscow, Atomizdat, 1964, pp. 137-148. (In Russian).

19. Volkenstein V. Biophysics. Moscow, Nauka, 1988. 431 p. (In Russian).

20. Kaprin A.D., Galkin V.N., Zhavoronkov L.P., Ivanov V.K., Ivanov S.A., Romanko Yu.S. Synthesis of basic and applied research is the basis for providing high-level scientific results and their introduction into medical practice. Radiatsiya i risk – Radiation and Risk, 2017, vol. 26, no. 2, pp. 26-40. (In Russian).

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