Analysis of TCR-mutant lymphocyte frequency in patients with colorectal cancers

«Radiation and Risk», 2013, vol. 22, No. 4, pp.14-20

Authors

Orlova N.V. – Senior Researcher, C. Sc., Biol., Medical Radiological Research Center of the Russian Ministry of Health, Obninsk. Contacts: 4 Korolyov str., Obninsk, Kaluga region, Russia, 249036. Tel.: (48439) 9-77-81; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Smirnova S.G. – Senior Researcher, C. Sc., Biol., Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.
Rukhadze G.O. – Research Assistant, Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.
Erygin D.V. – Senior Researcher, C. Sc., Med., Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.
Berdov B.A. – Deputy Director, MD, Prof., Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.
Lozebnoy N.I. – Postgraduate Student, Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.
Zamulaeva I.A. – Head of Lab., D.Sc., Biol., Prof., Medical Radiological Research Center of the Russian Ministry of Health, Obninsk.

Abstract

Comparative analysis of frequency of lymphocytes, bearing mutations at T-cell receptor locus (TCR), in patients with colorectal cancers was conducted. Frequency of mutant cells in patients prior to the treatment was significantly higher than that in control healthy persons (p=0.02). It was shown that 24% of patients before the treatment had increased level of mutant cells, it is comparable with the level in patients bearing malignant tumors of other localizations. No association of mutant cells frequency with stage of the disease and the localization of the primary tumor was found. The increased level of TCR-mutant lymphocytes in some patients with colorectal cancer before the treatment is supposed to be considered as prognostic marker of colorectal cancer risk.

Key words
Colorectal cancer, somatic mutagenesis, T-cell receptor, genome instability.

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