The early response of yeast to low doses of radiations with different LET

Issues

Authors

Tsyb T.S., Malinova I.V., Komarova E.V.
Medical Radiological Research Center of RAMS, Obninsk.

Abstract

The experiments were performed with the diploid wild strain Megry 139-B of yeast Saccharomyces. Monolayers of cells were irradiated with α-particles ²³⁹Pu (from 0.35 to 6.0 Gy), γ-rays (from 1.0 to 50 Gy) and neutrons (from 3.5 to 12 Gy). It was found that the kinetics of irradiated cells budding and morphology of microcolonies formed by these cells were changed after exposures with low doses of α-particles, neutrons and γ-rays. These alterations were revealed at the nearest descendants of irradiated cells (up to 8–10 generation).

Key words
Saccharomyces yeast cells, Meghri strain, small doses, gamma particles, ²³⁹Pu, neutrons, cell inactivation, budding process, reproductive death.

References

1. Evans H.J. Alpha-particle after effects. Nature. 1992. Vol. 355. PP. 674-675. No. 2. PP. 241-253.

2. Skov K.A. Radioresponsiveness at low doses: hyper-radiosensitivity and increased radioresistance in mammalian cells. Mutat. Res. 1999. Vol. 430, no. 2. PP. 241-253.

3. Jordan A., Laskowski W. Effects of low X-ray doses in Saccharomyces cerevisiae. Radiat. Environ. Biophys. 1987. Vol. 26. PP. 301-312.

4. Bychkovskaya I.B. The problem of distant radiation death of cells. Moscow, Energoatomizdat Publ., 1986. 160 c.

5. James A.P. Lethal Sectoring in yeast. Genetica. Proceedings of an International Workshop on the genetic Control of mutation, Texas. 1973. Suppl. PP. 165.

6. Dertinger H., Jung H. Molecular radiation biology. Berlin, Springer-Verlag, 1970.

7. Korogodin V.I. Problems of postradiation recovery. Moscow, Atomizdat Publ., 1966. 392 p.

8. Hall E.J. Radiobiology for the Radiologist, 3rd ed., pp. 169-177. Philadelphia, Lippincott, 1988.

9. Hei T.K., Нall E.J., Waldren C.A. Mutation induction and relative biological effectiveness of neutrons in mammalian cells. Radiat. Res. 1988. Vol. 115. PP. 281-291.

10. Yang T.C., Tobias C.A. Neoplastic cell transformation by energetic heavy ions and its modification with chemical agents. Adv. Space Res. 1984. Vol. 4. PP. 207-218.

11. Ritter M.A., Cleaver J.E., Tobias C.A. High-LET radiations induce a large proportion of non-rejoining DNA breaks. Nature (London). 1977. Vol. 266. P. 266.

12. Kadhim M.A. Radiation-induced genomic/chromosomal instability – recent advances in understanding this phenomenon. 31st Annual Meeting of the European Society for Radiation Biology. Dresden, Germany, 2001. P. 88.

13. Tsyb T.S. Morphological manifestations of the long-term consequences of gamma irradiation of yeast organisms. Reducing and compensatory processes in radiation injuries. Leningrad, 1982. pp. 91-92.

14. Fofanova K.A. Quantitative analysis of the reaction of the nuclear apparatus of yeast cells to irradiation. Journal of General Biology. 1967. Vol. 28, No. 1. PP. 116-121.

15. Tsyb T.S. et al. Enhancement of biological efficiency with the simultaneous action of electrons and alpha particles in comparison with sequential irradiation. Perspectives of medical application. VI International Scientific Conference. Sat. doc. TsNIIATOMINFORM. Zvenigorod, 2002. PP. 197-202.

16. Brooks A.L., Newton G.J., Shyr L.J. The combined effects of α-particles and X-rays on cell killing and micronuclei induction in lung epithelial cells. Int. J. of Radiat. Biol. 1990. Vol. 58, no. 5. PP. 799-811.

17. Nagasawa H. et al. Response of X-ray-sensitive CHO Mutant Cells (xrs-6c) to radiation. II. Relationship between cell survival and the induction chromosomal damage with low doses of α-particles. Radiat. Res. 1991. Vol. 126. PP. 280-288.

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