Forming groups of potential cancer risk from occupational exposure to radiation with the use of molecular and radiation epidemiological approaches

«Radiation and Risk», 2009, vol. 18, no. 2, pp.7-20

Authors

Ivanov V.K. – First Deputy Chairman of RSCRP, Deputy Director, Corr. Member of RAMS. Medical Radiological Research Center of the Russian Academy of Medical Sciences, Obninsk. Contacts: 4 Korolev str., Obninsk, Kaluga region, Russia, 249036. Tel.: (495) 956-94-12, (48439) 9-33-90; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Zamulaeva I.A. – Chief of Laboratory. Medical Radiological Research Center of the Russian Academy of Medical Sciences, Obninsk.
Kashcheeva P.V. – Postgraduate. Medical Radiological Research Center of the Russian Academy of Medical Sciences, Obninsk.
Saenko A.S. – Deputy Director, Professor. Medical Radiological Research Center of the Russian Academy of Medical Sciences, Obninsk.
Orlova N.B. – Senior Researcher. Medical Radiological Research Center of the Russian Academy of Medical Sciences, Obninsk.
Smirnova S.G. – Senior Researcher. Medical Radiological Research Center of the Russian Academy of Medical Sciences, Obninsk.
Korelo A.M. – Lead Programmer. Medical Radiological Research Center of the Russian Academy of Medical Sciences, Obninsk.
Gorsky A.I. – Lead Research Assistant, Cand.Sc., Tech. Medical Radiological Research Center of the Russian Academy of Medical Sciences, Obninsk.
Maksioutov M.А. – Chief of Laboratory, Cand.Sc., Tech. Medical Radiological Research Center of the Russian Academy of Medical Sciences, Obninsk.
Vaizer V.I. – Head Department of the Radiation Safety and Environmental Protection. State Scientific Center of the Russian Federation Institute for Physics and Power Engineering named after A.I.Leypunsky, Obninsk.

Abstract

In the study forming groups of potential cancer risk from occupational exposure to radiation with molecular and radiation epidemiological approaches is discussed. Frequency of gene mutations at TCR locus was measured in 320 professionals of the State Scientific Center of the Russian Federation Institute for Physics and Power Engineering named after A.I.Leypunsky. Average accumulated dose get by the workers was 100 mSv. Due to the use of radiation epidemiological approaches to defining the workers with the value of attributable radiation risk of 10 % and more it was managed to increase the portion of people with increased level of gene mutations in the group. It is noted that the obtained results are preliminary because of limited statistical power of studies.

Key words
Groups of potential cancer risk, occupational exposure, frequency of gene mutations at TCR locus, attributable risk.

References

1. Occupational health. Guidance on the assessment of occupational health risks for workers. Organizational and methodological foundations, principles and criteria for evaluation. P 2.2.1766-03. М: Ministry of Health of the Russian Federation, 2003.

2. Zamulaeva IA, Smirnova S.G., Orlova N.V. An increased frequency of mutant loci T-cell receptor and glycophorin A cells as a possible criterion for the formation of a risk group of oncological diseases. Russian Cancer Journal. 2001. No. 1. PP. 23-25.

3. Ivanov V.K., Panfilov A.P., Vasilenko E.K. et al. ARMIR: "international audit" of the state of ensuring radiation safety. ANRI. 2006. No. 4 (47). PP. 56-60.

4. Ivanov V.K., Tsyb A.F., Panfilov A.P., Agapov A.M. Optimization of radiation protection: "dose matrix". Moscow. Medicine Publ. 2006. 304 p.

5. Sayenko A.S., Zamulaeva I.A. Results and perspectives of the use of methods for determining the frequency of mutant cells at the loci of glycophorin A and T-cell receptor for assessing the genotoxic effect of ionizing radiation at remote times after exposure. Radiation Biology. Radioecology. 2000. Vol. 40, No. 5. PP. 549-553.

6. Sayenko A.S., Zamulaeva I.A., Smirnova S.G. Somatic mutagenesis in persons irradiated in small doses in the long term after exposure. Proceedings of the regional competition of scientific projects in the field of natural sciences. Kaluga: Eidos, 2003. Issue. 4. PP. 233-237.

7. Akiyama M., Kyoizumi S., Hirai Y. et al. Mutation frequency in human blood cells increases with age. Mutat. Res. 1995. Vol. 338. PP. 141-149.

8. Akiyama M., Umeki S., Kusunoki Y. et al. Somatic-cell mutation as a possible predictor of cancer risk. Health Phys. 1995. Vol. 68. PP. 643-649.

9. Breslow N.E., Day N.E. Statistical methods in cancer research. IARC Scientific publications. No. 32. Lyon, 1980. PP. 73-78.

10. Cole J., Scopek T.R. Somatic mutant frequency, mutation rates and mutational spectra in the human population in vivo. Mutat. Res. 1994. Vol. 304. PP. 33-105.

11. Dean A.G., Sullivan K.M., Soe M.M. OpenEpi: Open Source Epidemiologic Statistics for Public Health, Version 2.2.1. www.OpenEpi.com.

12. Grant B.W., Trombley L.M., Hunter T.C. et al. HPRT mutations in vivo in human CD34+ hemopoietic stem cells. Mutation Research. 1999. Vol. 431. PP. 183-198.

13. http://csrld.org.uk.

14. Ivanov V.K., Tsyb A.F., Agapov A.M. et al. Concept of optimization of the radiation protection system in the nuclear sector: management of individual cancer risks and providing targeted health care. Journal of Radiation Protection. 2006. Vol. 26. PP. 361-374.

15. Ivanov V.K., Tsyb A.F., Panfilov A.P. et al. Estimation of individualized radiation risk from chronic occupa-tional exposure in Russia. Health Physics. 2009. Vol. 97, No. 1. PP. 470-477.

16. Kyoizumi S., Akiyama M., Hirai Y. et al. Spontaneous loss and alteration of antigen receptor expression in mature CD4+ T cells. J. Exp. Med. 1990. Vol. 171. PP. 1981-1999.

17. Kyoizumi S., Kusunoki Y., Seyma T. et al. In vivo somatic mutations in Werner’s syndrome. Hum. Genet. 1998. Vol. 103, No. 4. PP. 405-410.

18. Kyoizumi S., Umeki S., Akiyama M. et al. Frequency of mutant T lymphocytes defective in the expression of the T-cell antigen receptor gene among radiation-exposed people. Mutat. Res. 1992. Vol. 265. PP. 173-180.

19. Land C., Gilbert E., Smith J.M. Report of the NCI-CDC Working Group to Revise the 1985 Radioepidemiological Tables, NIH Publication No. 03-5387. Washington, DC: US Department of Health and Human Services, 2003. 118 p.

Full-text article (in Russian)